Free lab tool · Australia
Peptide reconstitution calculator
Enter the mass of lyophilised peptide in the vial and the volume of bacteriostatic water you plan to add. The calculator returns the resulting concentration in mg/mL, the mass contained in each 0.01 mL graduation of a U-100 barrel, and the volume to draw for a target aliquot — the arithmetic every laboratory does by hand before preparing a working solution.
Your vial
Result
- Concentration
- 5 mg/mL
- Per mL
- 5,000 mcg
- Per 0.01 mL (1 unit, U-100 barrel)
- 50 mcg
- Volume to draw for target
- 0.05 mL(5 units)
- Aliquots per vial
- 40
Arithmetic only. Figures describe the concentration of a prepared laboratory solution and are not a dosing recommendation of any kind.
Method
How the calculation works
1. Concentration
Concentration (mg/mL) = peptide mass in the vial (mg) ÷ diluent volume (mL). Adding more water does not reduce the peptide present, it only spreads the same mass across a larger volume.
2. Per-graduation mass
Multiply mg/mL by 1000 to get mcg/mL, then divide by 100 because each unit on a U-100 barrel is 0.01 mL. That gives the mass sitting inside a single graduation.
3. Volume for a target
Volume (mL) = target mass (mcg) ÷ concentration (mcg/mL). Multiply by 100 to read the same volume as syringe units, then round to the nearest graduation you can measure accurately.
Questions
Reconstitution FAQ
How do you calculate peptide concentration after reconstitution?
Divide the mass of lyophilised peptide in the vial by the volume of diluent added. A 10 mg vial reconstituted with 2 mL of bacteriostatic water gives 10 ÷ 2 = 5 mg/mL, which is 5000 mcg per mL or 50 mcg per 0.01 mL graduation on a U-100 syringe.
How much bacteriostatic water should be added to a vial?
Any volume the vial can hold works chemically — the diluent volume only sets the concentration, never the total mass of peptide. Laboratories generally choose a volume that makes the aliquot they measure fall on a clear syringe graduation, most often 1 mL, 2 mL or 3 mL for a 10 mg vial.
What do insulin syringe units mean on this calculator?
A U-100 syringe is graduated so that 100 units equal 1 mL, meaning 1 unit equals 0.01 mL. The calculator converts your target mass into both millilitres and those unit graduations so a measured aliquot can be read directly off the barrel.
How should reconstituted material be stored?
Keep lyophilised vials at -20°C until use. Once reconstituted with bacteriostatic water, store refrigerated at 2–8°C, protect from light, and avoid repeated freeze-thaw cycles. Always confirm handling against the certificate of analysis for the specific lot.
Is this calculator dosing advice?
All products supplied by Pepper Labs are sold strictly as laboratory reference materials for in-vitro research and analytical use only. They are not medicines, not food, not cosmetics and not for human or veterinary consumption. Nothing on this website is medical advice, and no therapeutic claim is made or implied.
Research use only
Documented material, Australia wide
Pepper Labs supplies HPLC-verified research peptides with a certificate of analysis per lot, priced in AUD with flat $15 tracked shipping to every Australian address.