Free lab tool · Australia
Peptide reconstitution calculator
Your vial
Results
- Concentration
- 10 mg/mL
- Per mL
- 10 mg
- Per 0.01 mL (1 unit, U-100 barrel)
- 0.1 mg
- Volume to draw for target
- 0.05 mL(5 units)
- Total Doses Per Vial
- 20
Arithmetic only. Figures describe the concentration of a prepared laboratory solution and are not a dosing recommendation of any kind.
Syringe visualisation
5 units
01
Check your vial
Look at the label on your vial. Find the amount written in mg and enter that number into the first box.
02
Enter your water amount
Enter how many mL of bacteriostatic water you've added to your vial. This helps the calculator work out the concentration.
03
Enter your target amount
Enter your target amount in mg. The calculator automatically converts it into mL and U-100 syringe units.
04
Read your results
The results show the calculated volume and units. The gold marker on the syringe picture shows the corresponding measurement.
This calculator provides mathematical conversions only — it does not suggest or recommend doses.
Questions
Reconstitution FAQ
How do you calculate peptide concentration after reconstitution?
Divide the mass of lyophilised peptide in the vial by the volume of diluent added. A 10 mg vial reconstituted with 2 mL of bacteriostatic water gives 10 ÷ 2 = 5 mg/mL, which is 5000 mcg per mL or 50 mcg per 0.01 mL graduation on a U-100 syringe.
How much bacteriostatic water should be added to a vial?
Any volume the vial can hold works chemically — the diluent volume only sets the concentration, never the total mass of peptide. Laboratories generally choose a volume that makes the aliquot they measure fall on a clear syringe graduation, most often 1 mL, 2 mL or 3 mL for a 10 mg vial.
What do insulin syringe units mean on this calculator?
A U-100 syringe is graduated so that 100 units equal 1 mL, meaning 1 unit equals 0.01 mL. The calculator converts your target mass into both millilitres and those unit graduations so a measured aliquot can be read directly off the barrel.
How should reconstituted material be stored?
Keep lyophilised vials at -20°C until use. Once reconstituted with bacteriostatic water, store refrigerated at 2–8°C, protect from light, and avoid repeated freeze-thaw cycles. Always confirm handling against the certificate of analysis for the specific lot.
Is this calculator dosing advice?
All products supplied by Pepper Labs are sold strictly as laboratory reference materials for in-vitro research and analytical use only. They are not medicines, not food, not cosmetics and not for human or veterinary consumption. Nothing on this website is medical advice, and no therapeutic claim is made or implied.
Research use only
Documented material, Australia wide
Pepper Labs supplies HPLC-verified research peptides with a certificate of analysis per lot, priced in AUD with flat $15 tracked shipping to every Australian address.